Ethylene-enhanced ion and sucrose efflux in morning glory flower tissue

Journal Article · Tue Apr 01 04:00:00 EDT 1975 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.55.4.663· OSTI ID:7195136

Hanson, A D; Kende, H

Rib tissue segments excised from open flowers or buds of Ipomoea tricolor Cav. and floated on aqueous media responded to ethylene treatment by rolling up after 2 to 3 hours; a simple method for quantitating the rolling up is presented. The rolling up response was temperature and oxygen-dependent and was critically affected by the pH of the medium. The ethylene concentration giving a half-maximal response was 0.1 ..mu..l/l; continuous ethylene treatment was not required for the response as a 1-hour ethylene exposure enhanced rolling up. Rib segments rolling up during ethylene treatment unrolled when transferred to 0.5 M sucrose, indicating that rolling up was due to asymmetric turgor changes in the segments. Compartmental analysis of /sup 36/Cl- efflux from rib segments showed a fast and a slow phase; the slow phase, with a half-time of about 6 hours, is tentatively identified as efflux from the vacuolar compartment. During ethylene treatment, the rate of /sup 36/Cl- efflux in the slow phase rose markedly as the rolling up response developed. A similar result was obtained with the efflux of /sup 86/Rb/sup +/. The release of /sup 14/C-metabolites, labeled either by a period of /sup 14/CO/sub 2/ fixation in darkness or by exposure to /sup 14/C-(U)-glucose, also increased during ethylene, induced rolling up. These results suggest that ethylene causes an increase in membrane permeability in certain cells of the rib tissue. (auth)

• Research Organization: Michigan State Univ., East Lansing
• OSTI ID: 7195136
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 55:4; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ALKENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOMASS
BUDS
CARBON 14
CARBON ISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHLORINE 36
CHLORINE ISOTOPES
CRYOGENIC FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENERGY SOURCES
ETHYLENE
EVEN-EVEN NUCLEI
FLOWERS
FLUIDS
HYDROCARBONS
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
MEMBRANES
MINUTES LIVING RADIOISOTOPES
NONMETALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN
PERMEABILITY
PH VALUE
PLANTS
RADIOISOTOPES
RENEWABLE ENERGY SOURCES
RUBIDIUM 86
RUBIDIUM ISOTOPES
TEMPERATURE DEPENDENCE
YEARS LIVING RADIOISOTOPES