HCG-. beta. -subunit radioimmunoassay: potential error in HCG measurement related to choice of labeled antigen. [/sup 125/I]
Antiserum generated against the hormone-specific ..beta..-subunit of hCG was used with different labeled antigens to measure circulating hCG in patients having trophoblastic disease. When /sup 125/I-hCG..beta.. served as the labeled antigen, a small number of patient sera failed to show parallelism with the second IS-hCG reference and erroneous estimates of hormone concentrations were obtained. Replacement of the /sup 125/I-hCG..beta.. with labeled hCG corrected the nonparallelism exhibited by these samples. Inhibition curves obtained with purified hCG and hCG..beta.. suggested that both the nonparallelism and its correction with the change in labeled antigen would be consistent with the possibility that this assay aberration may result from the presence of free hCG..beta.. in these sera. (auth)
- Research Organization:
- Duke Univ. Medical Center, Durham, NC
- OSTI ID:
- 7195112
- Journal Information:
- Am. J. Obstet. Gynecol.; (United States), Vol. 125:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HCG
RADIOIMMUNOASSAY
BLOOD SERUM
ERRORS
IMMUNE SERUMS
IODINE 125
LABELLED COMPOUNDS
PATIENTS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GONADOTROPINS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics