Autologous immune responses to the major oncornavirus polypeptides in unmanipulated AKR/J mice

Journal Article · Thu Apr 01 04:00:00 EST 1976 · J. Virol.; (United States)

OSTI ID:7193069

Oldstone, M B.A.; Del Villano, B C; Dixon, F J

The autologous immune response of AKR/J mice to the structural proteins of murine leukemia virus (MuLV) was examined. Immunoglobulins from the renal glomeruli were chemically eluted, separated from antigens, recovered, and tested for immunological reactivity against MuLV structural proteins. Analyzing immune precipitates obtained after mixing radiolabeled Tween-disrupted MuLV preparations with eluates from AKR/J mice on sodium dodecyl sulfate-gel electrophoresis, we found evidence of antibodies to the major classes of MuLV structural components: gp70, gp45, p30, and one or more proteins in the 10,000- to 15,000-dalton class. Using rate zonal centrifugation we confirmed that the eluates from AKR/J glomeruli contained antibody(s) that bound specifically to p30. These results indicate that AKR/J mice spontaneously mount immune responses against the major oncornavirus polypeptide antigens.

• Research Organization: Scripps Clinic and Research Foundation, La Jolla, CA
• OSTI ID: 7193069
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 18:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
GLOBULINS
IMMUNE REACTIONS
IMMUNOGLOBULINS
MAMMALS
MICE
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RODENTS
VERTEBRATES
VIRUSES