Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities

Journal Article · Sun May 01 04:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.5.2237· OSTI ID:7192106

Swanson, M S; Dreyfuss, G

Several proteins of heterogeneous nuclear ribonucleoprotein (hnRNP) particles display very high binding affinities for different ribonucleotide homopolymers. The specificity of some of these proteins at high salt concentrations and in the presence of heparin allows for their rapid one-step purification from HeLa nucleoplasm. The authors show that the hnRNP proteins are poly(U)-binding proteins and compare their specificity to that of the previously described cytoplasmic poly(A)-binding protein. These findings provide a useful tool for the classification and purification of hnRNP proteins from various tissues and organisms and indicate that different hnRNP proteins have different RNA-binding specificities.

• Research Organization: Dept. of Biochemistry, Molecular Biology, and Cell Biology, Northwestern Univ., Evanston, IL (US)
• OSTI ID: 7192106
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:5; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201 -- Chemical & Physicochemical Properties
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANTICOAGULANTS
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CHEMICAL BONDS
CLASSIFICATION
DRUGS
HELA CELLS
HEMATOLOGIC AGENTS
HEPARIN
KINETICS
MUCOPOLYSACCHARIDES
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
PROTEINS
PURIFICATION
REACTION KINETICS
RNA
SACCHARIDES
SEPARATION PROCESSES
SPECIFICITY