Hypophysectomy eliminates and growth hormone (GH) maintains the midpregnancy elevation in GH receptor and serum binding protein in the mouse

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-126-2-1270· OSTI ID:7192079

Sanchez-Jimenez, F; Fielder, P J; Martinez, R R; Smith, W C; Talamantes, F ^[1]

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1. Univ. of California, Santa Cruz (USA)

({sup 125}I)Iodomouse GH (({sup 125}I)iodo-mGH) binding to samples of serum and hepatic microsomal membranes was measured in hypophysectomized pregnant, sham-operated pregnant, intact pregnant, and intact adult virgin mice. Surgeries were carried out on day 11 of pregnancy, and the animals were killed on day 14. The binding of mGH to both serum and hepatic microsomal membranes of intact virgin mice was much lower than to those of intact pregnant mice. In hypophysectomized mice, the mGH-binding capacity of both serum and hepatic microsomes decreased to values similar to those of nonpregnant mice. No significant differences were observed between intact and sham-operated pregnant animals in the maternal serum mGH concentration, the serum GH-binding protein concentration, or the hepatic GH receptor concentration. GH receptor and binding protein-encoding mRNAs were also higher in intact and sham-operated pregnant mice than in virgin and hypophysectomized mice. Hypophysectomized mice were treated with 200 micrograms/day bovine GH, administered by osmotic minipump; after 3 days of treatment, a significant elevation of hepatic GH receptor and serum GH-binding protein levels was observed. These results demonstrate an up-regulation of hepatic GH receptors and serum GH-binding protein by GH during pregnancy in the mouse.

• OSTI ID: 7192079
• Journal Information: Endocrinology; (USA), Vol. 126:2; ISSN 0013-7227
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
STH
RECEPTORS
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
DNA HYBRIDIZATION
IODINE 125
LIVER
MESSENGER-RNA
MICE
MICROSOMES
PREGNANCY
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HORMONES
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
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ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RIBOSOMES
RNA
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques