Heterogeneity of collagens in rabbit cornea: type III collagen

Journal Article · Sun May 01 04:00:00 EDT 1988 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:7191943

Cintron, C; Hong, B S; Covington, H I; Macarak, E J

Whole neonate rabbit corneas and adult corneas containing 2-week-old scars were incubated in the presence of (/sup 14/C) glycine. Radiolabeled collagen extracted from the corneas and scar tissue were analyzed by sodium dodecylsulfate/polyacrylamide gel electrophoresis and fluorography to determine the types and relative quantity of collagen polypeptides present and synthesized by these tissues. In addition to other collagen types, type III was found in both neonate cornea and scar tissue from adult cornea, albeit in relatively small quantities. Type III collagen in normal cornea was associated with the residue after pepsin digestion and formic acid extraction of the tissue, and the same type of collagen was extracted from scar tissue after similar treatment. Type III collagen-specific monoclonal antibody bound to developing normal corneas and healing adult tissue sections, as determined by immunofluorescence. Antibody binding was localized to the endothelium and growing Descemet's membrane in fetal and neonate corneas, and restricted to the most posterior region of the corneal scar tissue. Although monoclonal antibody to keratan sulfate, used as a marker for stromal fibroblasts, bound to most of the scar tissue, the antibody failed to bind to the posterior scar tissue positive for type III collagen. We conclude that endothelial cells from fetal and neonate rabbit cornea and endothelium-derived fibroblasts from healing wounds of adult cornea synthesize and deposit type III collagen. Moreover, this collagen appears to be incorporated into the growing Descemet's membrane of normal corneas and narrow posterior portion of the scar tissue.

• Research Organization: Eye Research Institute, Boston, MA (USA)
• OSTI ID: 7191943
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Journal Name: Invest. Ophthalmol. Visual Sci.; (United States) Vol. 29:5; ISSN IOVSD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RECOVERY
BODY
BODY AREAS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
COLLAGEN
CONNECTIVE TISSUE CELLS
CORNEA
ELECTROPHORESIS
ENDOTHELIUM
EYES
FACE
FIBROBLASTS
FLUORESCENCE
GLYCINE
HEAD
HEALING
INJURIES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LUMINESCENCE
MAMMALS
MEMBRANES
MONOCLONAL ANTIBODIES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RABBITS
REACTION KINETICS
RECOVERY
SCLEROPROTEINS
SENSE ORGANS
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
WOUNDS