Role for tryptophan in regulation of protein synthesis in porcine muscle
Experiments were conducted to determine the effect of varying concentrations of dietary tryptophan on growth rate and protein synthesis in edible muscle tissues of growing swine. A total of 45 immature swine (initial weight approximately 24 kg) were fed corn-gelatin diets containing 0.5 (n = 8), 0.8 (n = 10), 1.3 (n = 10), 1.5 (n = 7) or 2.0 (n = 10) g tryptophan/kg diet for 35 d. Animals fed 0.5 and 0.8 g tryptophan/kg grew more slowly, consumed less feed and had a lower efficiency of feed utilization than animals fed higher concentrations of tryptophan. Thirty similar animals were used in a second experiment. Diets containing 0.5, 0.8, 1.0, 1.5 or 2.0 g tryptophan/kg diet (n = 6) were fed for 14 d, after which all animals were killed and samples were taken of longissimus dorsi, triceps brachii and biceps femoris. Protein synthetic activity was determined by monitoring the incorporation of (/sup 14/C)phenylalanine into protein in vitro. There was no significant difference in synthetic activity between different muscle types. There was no effect of diet on the activity of the muscle soluble protein fraction. The activity of the muscle ribosomal fraction, however, was positively correlated with increasing concentrations of dietary tryptophan. It was concluded that tryptophan has the potential to regulate muscle protein synthesis in a manner beyond serving simply as a component of protein.
- Research Organization:
- Univ. of Guelph, Ontario (Canada)
- OSTI ID:
- 7191875
- Journal Information:
- J. Nutr.; (United States), Vol. 118:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Combined effect of electrical stimulation and blade tenderization on some bovine muscles
Comparative effect of oral ingestion of methyl mercury on chicks and rats
Related Subjects
PROTEINS
BIOSYNTHESIS
TRYPTOPHAN
BIOLOGICAL FUNCTIONS
CARBON 14 COMPOUNDS
MUSCLES
PHENYLALANINE
RIBOSOMES
SWINE
TRACER TECHNIQUES
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
AZOLES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DOMESTIC ANIMALS
FUNCTIONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PYRROLES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques