Effects of epidermal growth factor on bone formation and resorption in vivo

Journal Article · Thu Feb 01 00:00:00 EST 1990 · American Journal of Physiology; (USA)

OSTI ID:7191872

Marie, P J; Hott, M; Perheentupa, J ^[1]

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1. Institut National de la Sante et de la Recherche Medicale, Paris (France)

The effects of mouse epidermal growth factor (EGF) on bone formation and resorption were examined in male mice. EGF administration (2-200 ng.g-1.day-1 ip for 7 days) induced a dose-dependent rise in plasma EGF levels that remained within physiological range. Histomorphometric analysis of caudal vertebrae showed that EGF (20 and 200 ng.g-1.day-1) reduced the endosteal matrix and mineral appositional rates after 5 days of treatment as measured by double (3H)proline labeling and double tetracycline labeling, respectively. This effect was transitory and was not observed after 7 days of EGF administration. EGF administered for 7 days induced a dose-dependent increase in the periosteal osteoblastic and tetracycline double-labeled surfaces. At high dosage (200 ng.g-1.day-1) EGF administration increased the osteoclastic surface and the number of acid phosphatase-stained osteoclasts, although plasma calcium remained normal. The results show that EGF administration at physiological doses induces distinct effects on endosteal and periosteal bone formation and that the effects are dependent on EGF dosage and duration of treatment. This study indicates that EGF at physiological dosage stimulates periosteal bone formation and increases endosteal bone resorption in the growing mouse.

• OSTI ID: 7191872
• Journal Information: American Journal of Physiology; (USA), Vol. 258; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BONE CELLS
CELL DIVISION
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
ALKALINE PHOSPHATASE
AUTORADIOGRAPHY
CALCIUM
DOSE-RESPONSE RELATIONSHIPS
EPIDERMIS
KIDNEYS
MICE
PROLINE
TETRACYCLINES
TRITIUM COMPOUNDS
ALKALINE EARTH METALS
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZOLES
BODY
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DRUGS
ELEMENTS
ENZYMES
EPITHELIUM
ESTERASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROLASES
KINETICS
MAMMALS
METALS
MITOGENS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHATASES
PROTEINS
PYRROLES
PYRROLIDINES
REACTION KINETICS
RODENTS
SKIN
SOMATIC CELLS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques