Ascorbic acid: Nonradioactive extracellular space marker in canine heart

Journal Article · Sun Nov 01 04:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:7191772

Reil, G H; Frombach, R; Kownatzki, R; Quante, W; Lichtlen, P R ^[1]

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1. Medizinische Hochschule Hannover (West Germany)

The distribution pattern of ascorbic acid and L-({sup 14}C)ascorbic acid in myocardial tissue was compared with those of the classical radioactive extracellular space markers ({sup 3}H)-inulin, ({sup 3}H)sucrose, and Na{sup 82}Br. A new polarographic techniques was developed for analogue registration of ascorbic acid concentration in coronary venous blood. The kinetic data of the markers were studied in an open-chest canine heart preparation during a constant tracer infusion of up to 9 min. Distribution volumes were calculated based on the mean transit time method of Zierler. The distribution volume of ascorbic acid as well as of L-({sup 14}C)ascorbic acid in myocardial tissue agreed closely with those of ({sup 3}H)inulin and ({sup 3}H)sucrose as well as {sup 82}Br. The obtained kinetic data confirmed that ascorbic acid exhibits the physicochemical properties of an extracellular space marker, though this compound was shown to leak slowly into myocardial cells. Favorable attributes of this indicator are its low molecular weight, high diffusibility in interstitial fluid, low binding affinity to macromolecules, and high transcapillary as well as low transplasmalemmal penetration rate. Therefore, this nonradioactive marker can be applied in a safe and simple fashion, and without untoward side effects in experimental animals as well as in patients.

• OSTI ID: 7191772
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
ASCORBIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MARKERS
BODY
BROMIDES
BROMINE 82
BROMINE COMPOUNDS
BROMINE ISOTOPES
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISACCHARIDES
DISTRIBUTION
DOGS
HALIDES
HALOGEN COMPOUNDS
HEART
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
INULIN
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MUSCLES
MYOCARDIUM
NUCLEI
ODD-ODD NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PERFUSED TISSUES
POLYSACCHARIDES
RADIOISOTOPES
RADIONUCLIDE KINETICS
SACCHARIDES
SACCHAROSE
SODIUM BROMIDES
SODIUM COMPOUNDS
TISSUE DISTRIBUTION
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES
VITAMINS