Monoclonal approach to investigate whether the idiotypic network plays a role in the formation of Graves' autoantibodies
A monoclonal antibody (LE4) specific for a thyrotropin (TSH) epitope shared by beta subunits of bovine (b), ovine (o), and human (h) TSH was obtained by immunization of mice with mixtures of purified bTSH and hTSH. LE4 also bound the beta subunits of bovine, ovine, and human lutropin (LH), and human chorionic gonadotropin (hCG) but not the beta subunits of porcine LH and TSH. Preliminary studies with deglycosylated (dg) bTSH and dg-hCG indicated that part of the epitope may be carbohydrate. Preincubation of LE4 with (/sup 125/I)bTSH inhibited the binding of the hormone to TSH receptor of bovine thyroid membranes in a dose-dependent manner. LE4 also inhibited TSH-induced mitogenesis of FRTL-5 cells. We conclude that LE4 binds to a site on the bTSH molecule that participates in high affinity binding of hormone to TSH receptor.
- Research Organization:
- Columbia Univ., New York (USA)
- OSTI ID:
- 7191768
- Country of Publication:
- United States
- Language:
- English
Similar Records
Monoclonal antibodies to the thyrotropin receptor raised by an autoantiidiotypic protocol and their relationship to monoclonal autoantibodies from Graves' patients
Use of polyclonal and monoclonal antibodies to study hCG-receptor interactions
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CATTLE
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
RUMINANTS
SHEEP
SPECIFICITY
TRACER TECHNIQUES
TSH
VERTEBRATES