Monoclonal approach to investigate whether the idiotypic network plays a role in the formation of Graves' autoantibodies

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:7191768

Hill, B L

A monoclonal antibody (LE4) specific for a thyrotropin (TSH) epitope shared by beta subunits of bovine (b), ovine (o), and human (h) TSH was obtained by immunization of mice with mixtures of purified bTSH and hTSH. LE4 also bound the beta subunits of bovine, ovine, and human lutropin (LH), and human chorionic gonadotropin (hCG) but not the beta subunits of porcine LH and TSH. Preliminary studies with deglycosylated (dg) bTSH and dg-hCG indicated that part of the epitope may be carbohydrate. Preincubation of LE4 with (/sup 125/I)bTSH inhibited the binding of the hormone to TSH receptor of bovine thyroid membranes in a dose-dependent manner. LE4 also inhibited TSH-induced mitogenesis of FRTL-5 cells. We conclude that LE4 binds to a site on the bTSH molecule that participates in high affinity binding of hormone to TSH receptor.

• Research Organization: Columbia Univ., New York (USA)
• OSTI ID: 7191768
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MONOCLONAL ANTIBODIES
SPECIFICITY
TSH
BIOCHEMICAL REACTION KINETICS
RECEPTORS
CATTLE
DOSE-RESPONSE RELATIONSHIPS
IODINE 125
MAN
MICE
SHEEP
TRACER TECHNIQUES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
RUMINANTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques