Effect of swainsonine on processing and turnover of two lysosomal glycoproteins from mouse peritoneal macrophages
No effect of the inhibitor on the relative rates of synthesis of the precursor form of either enzyme was observed with either (/sup 35/S)methionine or 2-(/sup 3/H)mannose as the labeled tracer. On the other hand, processing of ..beta..-galactosidase and ..beta..-glucuronidase was markedly altered by swainsonine as manifested by a number of differences in the processed forms. (1) The altered forms had slightly greater electrophoretic mobility than normal forms. (2) Endo-..beta..-N-acetylglucosaminidase H digestion decreased the molecular sizes of the altered forms much more than those of the normal forms. (3) On isoelectric separation of the charge isoforms of ..beta..-glucuronidase, the three most negatively charged forms of the normal processed enzyme were absent in the altered material. This difference was eliminated by neuraminidase treatment of the processed form. These findings are consistent with a blockage by swainsonine of the removal of the ..cap alpha..-1,3 and ..cap alpha..-1,6 linked mannose residues that occurs in normal processing.
- Research Organization:
- State Univ. of New York, Buffalo (USA)
- OSTI ID:
- 7191731
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ENZYME INHIBITORS
BIOLOGICAL FUNCTIONS
GLUCOPROTEINS
METABOLISM
ELECTROPHORESIS
GALACTOSIDASE
GLUCURONIDASE
LYSOSOMES
MACROPHAGES
MANNOSE
METHIONINE
MICE
PERITONEUM
PRECURSOR
SULFUR 35
TRACER TECHNIQUES
ALDEHYDES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
FUNCTIONS
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MEMBRANES
MONOSACCHARIDES
NUCLEI
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
PHAGOCYTES
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SEROUS MEMBRANES
SOMATIC CELLS
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques