Extracellular nucleotide catabolism in human B and T lymphocytes. The source of adenosine production
Extracellular nucleotide degradation was studied in intact human B and T lymphocyte subpopulations and in lymphoblastoid cell lines. Cells of B lymphocyte lineage showed high nucleotide degrading activity, whereas T lymphocytes were unable to degrade extracellular nucleotides. The external surface of B cells contained active sites of ecto-triphosphonucleotidase (ecto-ATPase), ecto-diphosphonucleotidase (ecto-ADPase), and ecto-monophosphonucleotidase (ecto-AMPase). The expression of all three ectoenzyme activities seemed closely associated with B cell development. ATPase and ADPase activities increase continuously during B cell maturation, ecto-AMPase activity, on the other hand, reaches maximal activity in late pre-B cells. These results combined with our previous studies of intracellular ATP catabolism provide evidence that extracellular ATP catabolism may represent exclusive source for adenosine in lymphocytes. It is suggested that adenosine may serve as a means of communication between B and T cells in lymphoid organs, B lymphocytes being the sole producers of adenosine and T lymphocytes being the recipients of this signal.
- Research Organization:
- Hospital for Sick Children, Toronto, Ontario (Canada)
- OSTI ID:
- 7191476
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:15; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inhibitory effect of extracellular purine nucleotide and nucleoside concentrations on T cell proliferation
Extracellular adenosine triphosphate increases cation permeability of chronic lymphocytic leukemic lymphocytes
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ADENOSINE
ANIMAL CELLS
AROMATICS
ATP-ASE
AZAARENES
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATABOLISM
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROLASES
INOSINE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
METABOLISM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOHYDROLASES
PURINES
RIBOSIDES
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS