Biotransformation and detoxication of molinate (Ordram) in fish
Bioconcentration, deputation, and biotransformation of molinate were compared in common carp (cyprinus carpio), striped bass (Morone saxatilis), and white sturgeon (acipenser transmontanus) using a flow-through metabolism system. When compared to static conditions, flowing water improved oxygenation, decreased chemical volatilization and remetabolism, and run through a macroreticular resin, improved waste-product collection. Metabolite analysis employed gradient high-pressure liquid chromatography. Exposure to 100 ..mu..g L/sup -1/ (ring-/sup 14/C)molinate for 24 h resulted in bioconcentration factors of 30.5 (carp), 25.3 (bass), and 19.7 (sturgeon); differences were not significant (all, P > 0.05). /sup 14/C depuration by common carp was significantly slower than that by either striped bass or white sturgeon (both, P < 0.01). All three species oxidized molinate to a number of products and hydrolyzed, or conjugated with glutathione (GSH), the sulfoxide or sulfone, ultimately producing the mercapturic acid; carp and sturgeon also formed a D-glucuronic acid conjugate. Common carp were significantly less capable of sulfoxidation and GSH conjugation than either striped bass (P < 0.05) or white sturgeon (P < 0.01). Therefore, the selective toxicity of molinate in carp may be due to less efficient depuration and metabolic deactivation.
- Research Organization:
- California Univ., Davis (USA)
- OSTI ID:
- 7191362
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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XENOBIOTICS
METABOLISM
TOXICITY
CARBON 14 COMPOUNDS
CHEMICAL EFFLUENTS
FISHES
GENETIC VARIABILITY
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
TRACER TECHNIQUES
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL VARIABILITY
CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
SEPARATION PROCESSES
VERTEBRATES
550501* - Metabolism- Tracer Techniques