Studies by electron paramagnetic resonance spectroscopy of xanthine oxidase enriched with molybdenum-95 and with molybdenum-97

Journal Article · Tue May 17 04:00:00 EDT 1988 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00410a011· OSTI ID:7191265

George, G N; Bray, R C

Investigations have been carried out on the nature of the species from the enzyme xanthine oxidase that give rise to the two molybdenum(V) electron paramagnetic resonance (EPR) signals. Isotopic enrichment with /sup 95/Mo, /sup 97/Mo, /sup 33/S, and /sup 17/O was employed. Computer simulations of the EPR spectra recorded at 9- and 35-GHz microwave frequencies were used to evaluate the various hyperfine couplings and angular relations between the principal axes of g and A, as well as the nuclear electric quadrupole interaction for /sup 97/Mo. The results support the presence of an oxo ligand in the Rapid and of both an oxo and a sulfido ligand in the Very Rapid signal-giving species.

• Research Organization: Univ. of Sussex, Brighton (England)
• OSTI ID: 7191265
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:10; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COMPUTERIZED SIMULATION
DAYS LIVING RADIOISOTOPES
ELECTRON SPECTRA
ELECTRON SPIN RESONANCE
ENZYMES
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
MOLYBDENUM 95
MOLYBDENUM 97
MOLYBDENUM ISOTOPES
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN 17
OXYGEN ISOTOPES
PURINES
RADIOISOTOPES
RESONANCE
SIMULATION
SPECTRA
STABLE ISOTOPES
SULFUR 35
SULFUR ISOTOPES
XANTHINES