Enhanced thromboxane synthesis in atria from infarcted rabbit hearts

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7189313

Dunkel, C G; Evers, A S; Needleman, P

The authors have previously shown that left ventricular myocardial infarction (MI) results in enhanced thromboxane (Tx) synthesis in response to n-formylmethionyl-leucyl-phenylalanine (fMLP). To anatomically localize this response, cardiac atria and ventricles were removed from normal rabbits and rabbits subjected 4 days previously to MI. Atria were placed in a tissue bath, ventricles were perfused with buffer via the vasculature and both preparations were challenged with fMLP. TxB/sub 2/ in the bath media or ventricular effluent was measured by specific RIA. Atria from normal and infarcted hearts produced similar basal levels of Tx. Following fMLP stimulation, atria from infarcted hearts produced 10X more Tx than normal atria. Ventricles from normal and infarcted hearts produced no Tx basally and only small quantities with fMLP stimulation. Incubation of microsomes prepared from the various chambers of the heart with (/sup 14/-C) arachidonate showed that Tx synthetic capacity in both normal and infarcted hearts resides almost exclusively in the right cardiac atria. These results show that cardiac Tx synthesis is largely an atrial phenomenon and that left ventricular myocardial infarction results in enhanced fMLP-stimulated Tx synthesis.

• Research Organization: Washington Univ. Medical School, St. Louis, MO
• OSTI ID: 7189313
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DISEASES
HEART
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
MYOCARDIAL INFARCTION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PERFUSED ORGANS
PROSTAGLANDINS
RABBITS
SYNTHESIS
VERTEBRATES