In vitro evidence of cellular adaptation to ozone toxicity in the rat trachea

Journal Article · Sun May 01 04:00:00 EDT 1988 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(88)90042-7· OSTI ID:7189257

Nikula, K J; Wilson, D W; Dungworth, D L; Plopper, C G

Adaptation to prolonged ozone (O/sub 3/) exposure occurs in the tracheal epithelium of rats and is marked by the presence of ciliated cells with uniform short cilia but is not accompanied by shifts in cell populations, altered characteristics of epithelial secretory cell products, increased cell turnover, or elevated tracheal superoxide dismutase activity. The purpose of this study was to test the hypothesis that adaptation is a result of alterations intrinsic to epithelial cells or to the cells and their matrix, and not due to systemic or neural influences. Rats were preexposed to either filtered air (FA) or 0.96 ppm O/sub 3/ for 8 hr/night for 60 days, and then their tracheae were removed and exposed to 3 ppm O/sub 3/ in an explant culture system where behavioral, nasal, exudative, and secretory product influences can be eliminated. After exposure to 3 ppm O/sub 3/ in vitro, quantitative electron microscopic evaluation demonstrated that the epithelia from the FA preexposure group had significantly more necrotic cells and sloughed cells and fewer ciliated cells than the epithelia from the O/sub 3/ preexposure group. Thus previous exposure to ozone in vivo induces a change in tracheal epithelium which confers resistance to ozone-induced injury in the explant exposure system.

• Research Organization: Univ. of California, Davis (USA)
• OSTI ID: 7189257
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 93:3; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
ANIMAL TISSUES
ANIMALS
BIOLOGICAL ADAPTATION
BODY
DRUGS
ENZYME ACTIVITY
ENZYMES
EPITHELIUM
GLUTATHIONE
IN VITRO
MAMMALS
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OZONE
PEPTIDES
POLLUTION
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RESPIRATORY SYSTEM
RODENTS
SUPEROXIDE DISMUTASE
TISSUES
TOXICITY
TRACHEA
ULTRASTRUCTURAL CHANGES
VERTEBRATES