Manganese attenuates secretagogue-mediated phospholipid hydrolysis in AR42J cells
Journal Article
·
· Life Sciences; (USA)
- Univ. of Arizona College of Medicine, Tucson (USA)
The effects of cholecystokinin octapeptide (CCK8), bombesin and manganese (Mn{sup 2+}) on phosphatidylinositol-4,5-bisphosphate (PIP{sub 2}) hydrolysis were studied in AR42J cells. One-half maximal stimulation of inositol monophosphate (InsP{sub 1}) accumulation occurred at either 5 nM CCK8 or 5 nM bombesin, and maximal stimulation occurred at 30 nM for each agonist. Mn{sup 2+} did not alter basal PIP2 hydrolysis. However, addition of Mn{sup 2+} 5 min prior to stimulation with either CCK8 or bombesin for 60 min significantly attenuated ({sup 3}H)InsP1 accumulation. Following brief periods of incubation with CCK8 (15 sec) Mn{sup 2+} significantly reduced inositol tris- and tetrakisphosphate accumulation. These data suggest that Mn{sup 2+} may participate in the regulation of CCK8- and bombesin-mediated generation of phosphoinositides.
- OSTI ID:
- 7189057
- Journal Information:
- Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 45:21; ISSN 0024-3205; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
ELEMENTS
ESTERS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIPIDS
MAMMALS
MANGANESE
METABOLISM
METALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDES
PHOSPHOLIPIDS
PROTEINS
RATS
RODENTS
TRACER TECHNIQUES
TRANSITION ELEMENTS
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
ELEMENTS
ESTERS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIPIDS
MAMMALS
MANGANESE
METABOLISM
METALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDES
PHOSPHOLIPIDS
PROTEINS
RATS
RODENTS
TRACER TECHNIQUES
TRANSITION ELEMENTS
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES