Interspecies differences in the hepatic metabolism of 2,3,7,8-tetrachlorodibenzo-p-dioxin: role in toxicity

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:7189026

Wroblewski, V J

These studies examined the hepatic metabolism of TCDD in suspensions of isolated hepatocytes from the guinea pig and hamster. /sup 14/C- TCDD was metabolized at a similar rate in hepatocytes from the three species. TCDD pretreatment 72 hours prior to hepatocyte isolation increased cytochrome P-448-mediated monooxygenase activities and increased the rate of TCDD metabolism in rat and hamster hepatocytes 5-6-fold over the control rate. However, pretreatment of guinea pigs with TCDD had not effect on the rate of /sup 14/C-TCDD metabolism in isolated hepatocytes from this species. The findings suggest that the inability of TCDD to induce its own rate of hepatic metabolism in the guinea pig may contribute to the persistence of TCDD and unique sensitivity of this species to the acute toxicity of TCDD.

• Research Organization: State Univ. of New York, Buffalo (USA)
• OSTI ID: 7189026
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL VARIABILITY
CARBON 14 COMPOUNDS
DIOXIN
ENZYME ACTIVITY
ENZYMES
GENETIC VARIABILITY
GUINEA PIGS
HAMSTERS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
RATS
RODENTS
SOMATIC CELLS
TOXICITY
TRACER TECHNIQUES
VERTEBRATES