Comparison of oleic acid metabolism in the soybean (Glycine max (L. ) Merr. ) genotypes Williams and A5, a mutant with decreased linoleic acid in the seed

Journal Article · Thu May 01 04:00:00 EDT 1986 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.81.1.41· OSTI ID:7188952

Martin, B A; Rinne, R W

The metabolism of oleoyl coenzyme A (CoA) was examined in developing seed from two soybean (Glycine max (L.) Merr.) genotypes: Williams, a standard cultivar and A5, a mutant containing nearly twice the oleic acid (18:1) content of Williams. The in vitro rates of esterification of oleoyl-CoA to lysophosphatides by acyl-CoA: lysophosphatidylcholine acyltransferase was similar in both genotypes and lysophosphatidyl-ethanolamine was a poor substrate. Crude extracts desaturated exogenous (1-/sup 14/C)dioleoyl phosphatidylcholine at 14% of the rate achieved with (1-/sup 14/C)oleoyl-CoA, and 50 micromolar lysophosphaatidylcholine. The desaturase enzyme also required NADH for full activity. Extracts from Williams contained 1.5-fold more oleoyl phosphatidylcholine desaturase activity, on a fresh weight basis, than did A5 and appeared to have a similar affinity for oleoyl-CoA. There was 1.2- to 1.9-fold more linoleic acid (18:2) in phosphatidylcholine from Williams than from A5, measured at two stages of development, but both genotypes had a similar distribution of fatty acids in the one and two positions. Phosphatidylethanolamine in A5 contained relatively more linoleic acid (18:2) in the one position than did Williams. The increased oleic acid (18:1) content in A5 appeared to be a result of decreased rates of 18:1 desaturation of oleoyl-phosphatidylcholine in this genotype.

• Research Organization: Pioneer Hi-Bred International, Inc., Johnston, IA
• OSTI ID: 7188952
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 81:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
BACTERIA
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COENZYMES
ENZYME ACTIVITY
ESTERS
GLYCINE HISPIDA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
LIPIDS
METABOLISM
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MUTANTS
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHYSIOLOGY
PLANTS
RHIZOBIUM
SEEDS
TRACER TECHNIQUES