Introns in the genome of bacteriophage T4
RNA from T4-infected cells yields multiple end-labeled species when incubated with (..cap alpha..-/sup 32/P)GTP under self-splicing conditions. One of these corresponds to the previously characterized intron from the T4 td gene and, as shown in this work, the others represent additional group I introns in T4. Two loci distinct from the td gene were found to hybridize to the mixed GTP-labeled T4 RNA probe. These were mapped to the unlinked genes nrdB and sunY. Cloned DNA from the nrdB region that contained the intron was shown to generate characteristic group I splice products with RNA synthesized in vivo or in vitro. The splice junction of the nrdB gene was determined and the nature of the RNA reaction products characterized. In vivo expression of the nrdB gene and the open reading frame within the intron was studied using in-frame lacZ fusions and primer extension analyses. The data suggest that expression of the intron open reading frame is highly regulated during T4 infection. Possible regulatory mechanisms are discussed.
- Research Organization:
- State Univ. of New York, Albany (USA)
- OSTI ID:
- 7188055
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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GENETIC MAPPING
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BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
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550201* - Biochemistry- Tracer Techniques