Pt anti-cancer drug interactions with oligodeoxyribonucleotides
The Pt adducts of d(TGGT) were investigated by /sup 31/P and /sup 1/H NMR spectroscopy with the following compounds: cisPtA/sub 2/Cl/sub 2/ (A/sub 2/ = en, (NH/sub 3/)/sub 2/, (MeNH/sub 2/)/sub 2/, tn, Me/sub 2/ tn, and N,N-Me/sub 2/en) and transPt (NH/sub 3/)/sub 2/Cl/sub 2/. Limited studies were performed with d(TTGG), D(GGTT), D(pGGTT), and d(TAGT). For d(TGGT)Pt(en) and d(TGGT)cisPt(MeNH/sub 2/)/sub 2/, the downfield /sup 31/P NMR signal was assigned to the GpG moiety by selective 2D NMR techniques. It was demonstrated that Pt formed a crosslink with the GpG moiety and the G's were in a head-to-head configuration. A downfield /sup 31/P NMR signal appears to be characteristic of Pt-crosslinked species and can be correlated with potential hydrogen bonding ability of the Pt complexes and the oligonucleotides. The signal was not shifted as far downfield when the group cis to the 5' G was incapable of hydrogen bonding or when no phosphate group was 5' to the GpG moiety.
- Research Organization:
- Emory Univ., Atlanta, GA (USA)
- OSTI ID:
- 7188009
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTINEOPLASTIC DRUGS
BIOCHEMICAL REACTION KINETICS
NUCLEOTIDES
CHEMICAL SHIFT
NMR SPECTRA
PHOSPHORUS 31
PLATINUM
TRACER TECHNIQUES
DRUGS
ELEMENTS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PLATINUM METALS
REACTION KINETICS
SPECTRA
STABLE ISOTOPES
TRANSITION ELEMENTS
550201* - Biochemistry- Tracer Techniques