Automated film reader for DNA sequencing based on homomorphic deconvolution

Journal Article · Wed Jun 01 00:00:00 EDT 1994 · IEEE Transactions on Bio-Medical Engineering (Institute of Electrical and Electronics Engineers); (United States)

DOI:https://doi.org/10.1109/10.293238· OSTI ID:7172776

Ives, J T; Stockham, Jr, T G ^[1]; Gesteland, R F ^[2]

---

1. Univ. of Utah, Salt Lake City, UT (United States)
2. Howard Hughes Medical Inst., Salt Lake City, UT (United States)

An automated reader for electrophoresis based DNA sequencing methods is described that provides fast and accurate sequence determination. Digitized sequencing lanes are processed with homorphic blind deconvolution in preparation for peak detection, interlane alignment, peak refinement and base calling. Initial reads from direct blot sequencing films have error rates of about 1% at the rate of 5 nucleotides/s. Typical read lengths are 500-600 nucleotides. The described reader is a significant improvement over existing readers and could be an essential component in the sequencing efforts of the Human Genome Project. 31 refs., 9 figs.

• DOE Contract Number: FG02-88ER60700
• OSTI ID: 7172776
• Journal Information: IEEE Transactions on Bio-Medical Engineering (Institute of Electrical and Electronics Engineers); (United States), Journal Name: IEEE Transactions on Bio-Medical Engineering (Institute of Electrical and Electronics Engineers); (United States) Vol. 41:6; ISSN IEBEAX; ISSN 0018-9294
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AUTOMATION
DNA SEQUENCING
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EVALUATION
IONIZING RADIATIONS
LABELLED COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOTOGRAPHIC FILMS
RADIATIONS
STRUCTURAL CHEMICAL ANALYSIS
X RADIATION