Regulation of atrial natriuretic peptide receptors in cultured vascular smooth muscle cells of rat

Journal Article · Wed Jul 16 04:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)90296-2· OSTI ID:7171525

Hirata, Y; Takata, S; Takagi, Y; Matsubara, H; Omae, T

To elucidate the regulation of vascular receptors for atrial natriuretic peptide (ANP), they have studied the binding capacity of /sup 125/I-labeled rat (r) ANP using cultured vascular smooth muscle cells from rat aorta. After preincubation with 3.2 x 10/sup -8/M rANP at 37/sup 0/C, the binding capacity decreased as a function of time; the maximal receptor loss (70-75%) occurred after 4 hrs and persisted for 24 hrs. Pretreatment with cycloheximide (20 ..mu..g/ml) and actinomycin D (2 ..mu..g/ml) similarly caused a dramatic reduction (approx. 80%) of the binding capacity after 24 hrs; the half-life (t/sub 1/2/) of the receptor loss was approx. 7-8 hrs. Following removal of rANP, the down-regulated ANP receptors fully recovered in the presence of 10% fetal calf serum, but not in combination with either actinomycin D or cycloheximide. Concanavalin A dose-dependently inhibited the binding. The binding capacity also decreased with time in the presence of tunicamycin (1 ..mu..g/ml) with t/sub 1/2/ of approx. 30 hrs.

• Research Organization: National Cardiovascular Center Research Institute, Osaka, Japan
• OSTI ID: 7171525
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 138:1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTINOMYCIN
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIBODIES
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD SERUM
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CATTLE
CONCANAVALIN
CYCLOHEXIMIDE
DATA
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
FUNGICIDES
HEMAGGLUTININS
HORMONES
INFORMATION
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LECTINS
MAMMALS
MEMBRANE PROTEINS
MUSCLES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PESTICIDES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
RUMINANTS
TIME DEPENDENCE
TRACER TECHNIQUES
VERTEBRATES