Cytolytic pore-forming protein associated with the surface membrane of Naegleria fowleri
Thesis/Dissertation
·
OSTI ID:7170516
Whole cell homogenates of Naegleria fowleri were examined by hemolytic and /sup 51/Cr-release assays for the presence of cytolytic molecules which may participate in the cytopathogenic action of this amoeba. Two distinct cytolytic activities were found. A surface membrane cytolysin was identified which was found to be avidly associated with membranes possessing an equilibrium density of 1.135 g/cm/sup 3/ in isopycnic sucrose gradients. The activity of the surface membrane cytolysin was not affected by heating at 75/sup 0/C for 30 min. The second cytolytic activity was found in putative lysosomes possessing an equilibrium density of 1.162 g/cm/sup 3/ and was completely inactivated by heating at 75/sup 0/C for 30 min. Cytolysis produced in the presence of both cytolysins was consistently synergistic with respect to the activity of either cytolysin alone. The lesions produced on erythrocytes by this cooperative process were characterized by electron microscopy as transmembrane pores resembling a number of other cytolytic effector molecules including the ninth component of complement, perforins of cytolytic T lymphocytes, and the alphatoxin of Staphylococcus aureus.
- Research Organization:
- Miami Univ., Coral Gables, FL (USA)
- OSTI ID:
- 7170516
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMOEBA
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMIUM 51
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ERYTHROCYTES
EVEN-ODD NUCLEI
HOMOGENATES
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
ISOTOPES
LYSIS
MATERIALS
MEMBRANES
MICROORGANISMS
MICROSCOPY
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
PROTOZOA
RADIOASSAY
RADIOISOTOPES
SARCODINA
TEMPERATURE EFFECTS
62 RADIOLOGY AND NUCLEAR MEDICINE
AMOEBA
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMIUM 51
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ERYTHROCYTES
EVEN-ODD NUCLEI
HOMOGENATES
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
ISOTOPES
LYSIS
MATERIALS
MEMBRANES
MICROORGANISMS
MICROSCOPY
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
PROTOZOA
RADIOASSAY
RADIOISOTOPES
SARCODINA
TEMPERATURE EFFECTS