Cellular and molecular aspects of early bone development in the chick embryonic tibia
Mid-diaphyseal periosteal collars and the corresponding cartilage core were micro-dissected free from chick tibias and separately digested with a trypsin-collagenase enzyme mixture. The released cell populations were cultivated in vitro and characterized by morphological analysis, histochemical localization, of alkaline phosphatase, alizarin red S staining for mineral deposition, growth rate ((/sup 3/H)thymidine incorporation) and proteoglycan content. Results of these studies showed that periosteal collar cell cultures form nodule-like structures that stain positively with alkaline phosphatase and alizarin red S. Light and electron microscopic observation revealed cell and matrix morphologies similar to that of intact periosteum. The nodules were composed of plump cell types embedded within a mineralized matrix surrounded by a fibroblastic cell layer. Core cartilage cell cultures displayed typical characteristics of the hypertrophic state in their visual appearance and proteoglycan composition. The formation of osseous-like structures in periosteal collar cell cultures but not in core chondrocyte cell cultures demonstrates the relatively autonomous nature of periosteal ossification.
- Research Organization:
- Case Western Reserve Univ., Cleveland, OH (USA)
- OSTI ID:
- 7170342
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE CELLS
GROWTH
MORPHOLOGY
EMBRYOS
MINERALIZATION
ALIZARIN
ALKALINE PHOSPHATASE
CARTILAGE
CELL CULTURES
CHICKENS
DIGESTION
ELECTRON MICROSCOPY
EXPERIMENTAL DATA
FIBROBLASTS
IN VITRO
THYMIDINE
TIBIA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPSIN
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTHRAQUINONES
AROMATICS
AZINES
BIRDS
BODY
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DATA
DYES
ENZYMES
ESTERASES
FOWL
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROSCOPY
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PHOSPHATASES
PYRIMIDINES
QUINONES
REAGENTS
RIBOSIDES
SERINE PROTEINASES
SKELETON
SOMATIC CELLS
TISSUES
VERTEBRATES
550801* - Morphology- Tracer Techniques