Human endomembrane H sup + pump strongly resembles the ATP-synthetase of Archaebacteria

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.16.6067· OSTI ID:7168442

Suedhof, T C; Stone, D K; Johnston, P A; Xie, Xiaosong ^[1]; Fried, V A ^[2]

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1. Univ. of Texas Southwestern Medical Center, Dallas (USA)
2. Saint Jude Children's Research Hospital, Memphis, TN (USA)

Preparations of mammalian H{sup +} pumps that acidify intracellular vesicles contain eight or nine polypeptides, ranging in size from 116 to 17 kDa. Biochemical analysis indicates that the 70- and 58-kDa polypeptides are subunits critical for ATP hydrolysis. The amino acid sequences of the major catalytic subunits (58 and 70 kDa) of the endomembrane H{sup +} pump are unknown from animal cells. The authors report here the complete sequence of the 58-kDa subunit derived from a human kidney cDNA clone and partial sequences of the 70- and 58-kDa subunits purified from clathrin-coated vesicles of bovine brain. The amino acid sequences of both proteins strongly resemble the sequences of the corresponding subunits of the vacuolar H{sup +} pumps of Archaebacteria, plants, and fungi. The archaebacterial enzyme is believed to use a H{sup +} gradient to synthesize ATP. Thus, a common ancestral protein has given rise to a H{sup +} pump that synthesizes ATP in one organism and hydrolyzes it in another and is highly conserved from prokaryotes to humans. The same pump appears to mediate the acidification of intracellular organelles, including coated vesicles, lysosomes, and secretory granules, as well as extracellular fluids such as urine.

• OSTI ID: 7168442
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:16; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ACIDIFICATION
AMINO ACID SEQUENCE
ANIMALS
ATP-ASE
BARYONS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DNA
DNA SEQUENCING
ELEMENTARY PARTICLES
ENZYMATIC HYDROLYSIS
ENZYMES
FERMIONS
HADRONS
HYDROLASES
HYDROLYSIS
LIGASES
LYSIS
LYSOSOMES
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHOHYDROLASES
PRIMATES
PROTEINS
PROTONS
RECOMBINANT DNA
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES