Characterization of the interaction between recombinant human interferon-gamma and its receptor on human polymorphonuclear leukocytes

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Journal of Leukocyte Biology; (USA)

OSTI ID:7168416

Hansen, B D; Finbloom, D S ^[1]

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1. Walter Reed Army Institute of Research, Washington, DC (USA)

The interaction of human recombinant interferon-gamma (rIFN-gamma) with human polymorphonuclear cells (PMN) was investigated. Bolton-Hunter radioiodinated rIFN-gamma bound to PMN in a specific and saturable manner. Eleven hundred binding sites were observed with a Ka of 0.56 x 10(10) M-1. Binding to PMN was rapid with a K1 of 9 x 10(5) M-1 sec-1 at 4{degree}C. At 37{degree}C binding was complete within 6 min. About 50% of bound ligand was internalized within 30 min at 37{degree}C. The receptor demonstrated moderate lability at 37{degree}C in culture. After 1 h at 37{degree}C, PMN lost 80% of their {sup 125}I-rIFN-gamma binding sites. This loss was reversed in part by the presence of interleukin-1 in the culture, but not tumor necrosis factor. These studies provide a framework for further investigation into the signalling process of rIFN-gamma on PMN.

• OSTI ID: 7168416
• Journal Information: Journal of Leukocyte Biology; (USA), Journal Name: Journal of Leukocyte Biology; (USA) Vol. 47:1; ISSN JLBIE; ISSN 0741-5400
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GROWTH FACTORS
IN VITRO
INTERFERON
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MITOGENS
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
VERTEBRATES