Studies of interactions between the receptor for immunoglobulin E and other cellular components during signal transduction

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:7168249

Estes, K A.S.

The high affinity receptor for immunoglobulin E (IgE) on rat basophilic leukemia (RBL) cells mediates antigen-triggered cellular degranulation. As a first step in developing a reconstitution system to test the structural requirements of receptors for triggering cellular degranulation, polyethylene glycol-induced membrane fusion methods were used to introduce exogenous IgE receptors into living RBL cells. In cell-cell fusion experiments, RBL cells with rat IgE bound to receptors and containing (5-1,2-{sup 3}H(N))hydroxytryptamine binoxalate (({sup 3}H)5HT) in their secretory granules were fused to cells with receptors occupied by anti-dinitrophenyl (DNP) mouse IgE. ({sup 3}H)5HT release could be triggered specifically by multivalent DNP antigen. In vesicle-cell fusion experiments, plasma membrane vesicles with receptors occupied by anti-DNP mouse IgE were fused with RBL cells, and DNP antigen was found to trigger ({sup 3}H)5HT release. Receptors for IgE in reformed vesicles, prepared by solubilizing vesicles and removing the detergent, could also be fused into cells and mediate the stimulation of ({sup 3}H)5HT release.

• Research Organization: Cornell Univ., Ithaca, NY (USA)
• OSTI ID: 7168249
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL MEMBRANES
DISEASES
GLOBULINS
HEMIC DISEASES
HYDROGEN COMPOUNDS
IMMUNE SYSTEM DISEASES
IMMUNOGLOBULINS
ISOTOPE APPLICATIONS
KINETICS
LEUKEMIA
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NEOPLASMS
ORGANIC COMPOUNDS
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES