Immunomodulation of vascular endothelium: Effects of ultraviolet B irradiation on vein allograft rejection

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Journal of Vascular Surgery; (USA)

DOI:https://doi.org/10.1067/mva.1990.17037· OSTI ID:7167610

Marin, M L; Hardy, M A; Gordon, R E; Reemtsma, K; Benvenisty, A I ^[1]

---

1. Columbia Univ. College of Physicians and Surgeons, New York, NY (USA)

Prosthetic grafts of vein allografts are inadequate as small-diameter vessel substitutes. We have applied ultraviolet B (UVB) irradiation to modulate the immunogenicity of vein allografts to avoid immunologic injury. The veins of male ACI rats were irradiated with UVB (60 mJ/cm2) in situ and transplanted to male ACI rats (autografts) and female Lewis rats (allografts). Nonirradiated veins served as controls. At 4, 7, 14, and 28 days, all grafts were patent and were studied for morphologic changes by scanning electron microscopy and for immunogold labeling of major histocompatibility complex class II antigen expression. In autografts, scanning electron microscopy demonstrated minimal endothelial loss after grafting, regardless of UVB irradiation. Untreated allografts showed severe endothelial injury 4, 7, and 14 days after transplantation. UVB irradiation of veins protected allografts from injury to the endothelium and basement membrane. Major histocompatibility complex class II-positive endothelial cells were not seen in autografts but were seen in 40% of cells 4 days after transplantation in untreated allografts. UVB-treated allografts showed MHC class II antigen expression labeling of 20% of the endothelial cells. Barr body analysis demonstrated the donor origin of these endothelial cells. UVB irradiation of rat vein allografts prolongs endothelial survival while decreasing endothelial surface expression of class II antigens. These data suggest that modification of vein immunogenicity with UVB irradiation may permit functional survival of small-vessel allografts without chronic immunosuppression.

• OSTI ID: 7167610
• Journal Information: Journal of Vascular Surgery; (USA), Journal Name: Journal of Vascular Surgery; (USA) Vol. 11:1; ISSN 0741-5214; ISSN JVSUE
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABDOMEN
ANIMAL TISSUES
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD VESSELS
BODY
BODY AREAS
CARDIOVASCULAR SYSTEM
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
ENDOTHELIUM
GRAFTS
IMMUNITY
MAMMALS
MICROSCOPY
MORPHOLOGICAL CHANGES
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RATS
RODENTS
SCANNING ELECTRON MICROSCOPY
TISSUES
TRANSPLANTS
ULTRAVIOLET RADIATION
VEINS
VERTEBRATES