DNA polymerase. delta. and DNA repair: DNA repair synthesis in human fibroblasts requires DNA polymerase. delta
Thesis/Dissertation
·
OSTI ID:7167559
When UV-irradiated cultured diploid human fibroblasts were permeabilized with Brij-58 then separated from soluble material by centrifugation, conservative DNA repair synthesis could be restored by a soluble factor obtained from the supernate of similarly treated HeLa cells. Monoclonal antibody to KB cell DNA polymerase {alpha}, while binding to HeLa DNA polymerase {alpha}, did not bind to the HeLa DNA polymerase {delta}. Moreover, at micromolar concentrations N{sup 2}-(p-n-butylphenyl)-2{prime}-deoxyguanosine 5{prime}-triphosphate (BuPdGT) and 2(p-n-butylanilino)-2{prime}-deoxyadenosine 5{prime}-triphosphate (BuAdATP) were potent inhibitors of DNA polymerase {alpha}, but did not inhibit the DNA polymerase {delta}. Neither purified DNA polymerase {alpha} nor {beta} could promote repair DNA synthesis in the permeabilized cells. Furthermore, if monoclonal antibodies to DNA polymerase {alpha} BuPdGTP, or BuAdATP was added to the reconstituted system, there was no significant inhibition.
- Research Organization:
- California Univ., Berkeley, CA (USA)
- OSTI ID:
- 7167559
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DNA POLYMERASES
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
HELA CELLS
KINETICS
MAMMALS
MAN
MONOCLONAL ANTIBODIES
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
SOMATIC CELLS
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DNA POLYMERASES
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
HELA CELLS
KINETICS
MAMMALS
MAN
MONOCLONAL ANTIBODIES
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
SOMATIC CELLS
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES