Partial purification of gibberellin oxidases from spinach leaves. [Spinacia oleracea L]

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Plant Physiol.; (United States)

OSTI ID:7166078

Gilmour, S J; Bleecker, A B; Zeevaart, J A.D.

Four enzyme activities catalyzing the following oxidative steps in the gibberellin (GA) biosynthetic pathway have been extracted from spinach (Spinacia oleracea L.) leaves after exposure to 8 long days: GA/sub 12/ ..-->.. GA/sub 53/ ..-->.. GA/sub 44/ ..-->.. GA/sub 19/ ..-->.. GA/sub 20/. Two of these, GA/sub 53/ oxidase and GA/sup 19/ oxidase, were separable from the other two, GA/sub 44/ oxidase and GA/sub 12/ 13-hydroxylase, by anion exchange high performance liquid chromatography (HPLC). Apparent molecular weights of the four enzymes as determined by gel filtration HLPL are: GA/sub 12/ 13-hydroxylase, 28,400; GA/sub 43/ oxidase, 42,500; GA/sub 44/ oxidase, 38,100; GA/sub 19/ oxidase, 39,500. GA/sub 44/ oxidase was purified approximately 100-fold in 0.3% yield by a combination of ammonium sulfate fractionation, anion exchange HPLC, phenyl-Sepharose chromatography and gel filtration HLPC.

• Research Organization: Michigan State Univ., East Lansing (USA)
• DOE Contract Number: AC02-76ER01338
• OSTI ID: 7166078
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 85:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

500200* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ENZYME ACTIVITY
ENZYMES
ESTERS
FOOD
GIBBERELLIC ACID
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
LACTONES
LEAVES
LIQUID COLUMN CHROMATOGRAPHY
MOLECULAR WEIGHT
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
PURIFICATION
SEPARATION PROCESSES
SPINACH
SYNTHESIS
VEGETABLES