Regulation of carbon and electron flow in Propionispira arboris: Relationship of catabolic enzyme levels to carbon substrates fermented during propionate formation via the methylmalony coenzyme a pathway
- Univ. of Wisconsin, Madison (USA)
- Michigan State Univ., East Lansing (USA)
A detailed study of the glucose fermentation pathway and the modulation of catabolic oxidoreductase activities by energy sources (i.e., glucose versus lactate of fumarate) in Propionispira arboris was performed. {sup 14}C radiotracer data show the CO{sub 2} produced from pyruvate oxidation comes exclusively from the C-3 and C-4 positions of glucose. Significant specific activities of glyceraldehyde-3-phosphate dehydrogenase and fructose-1,6-bisphosphate aldolase were detected, which substantiates the utilization of the Embden-Meyerhoff-Parnas path for glucose metabolism. The methylmalonyl coenzyme A pathway for pyruvate reduction to propionate was established by detection of significant activities of methylmalonyl coenzyme A transcarboxylase, malate dehydrogenase, and fumarate reductase in cell-free extracts and by {sup 13}C nuclear magnetic resonance spectroscpic demonstation of randomization of label from (2-{sup 13}C)pyruvate into positions 2 and 3 of propionate. The specific activity of pyruvate-ferredoxin oxidoreductase, malate dehydrogenase, fumarate reductase, and transcarboxylase varied significantly in cells grown on different energy sources. D-Lactate dehydrogenase (non-NADH linked) was present in cells of P. arboris grown on lactate but not in cells grown on glucose or fumarate. These results indicate that growth substrates regulate synthesis of enzymes specific for the methylmalonyl coenzyme A path initial substrate transformation.
- DOE Contract Number:
- FG02-85ER13376
- OSTI ID:
- 7165151
- Journal Information:
- Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 170:9; ISSN JOBAA; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BACTERIA
BIOCONVERSION
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBON 13
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON ISOTOPES
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FERMENTATION
GLUCOSE
GROWTH
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAGNETIC RESONANCE
METABOLISM
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROPIONIC ACID
RESONANCE
SACCHARIDES
STABLE ISOTOPES
SUBSTRATES
TRACER TECHNIQUES