Characterization of C-Ki- und ras oncogenes in liver neoplasms of winter flounder from Boston Harbor

Conference · Thu Sep 01 04:00:00 EDT 1988 · Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA)

OSTI ID:7164673

McMahon, G; Huber, L J; Wogan, G N ^[1]; Moore, M J; Stegeman, J J ^[2]

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1. Massachusetts Institute of Technology, Cambridge (USA)
2. Woods Hole Oceanographic Institution, MA (USA)

Recently, it has been shown that cellular proto-oncogenes can be activated by single-base mutations. In addition, there is evidence to suggest that the nature of the activating mutation may be a consequence of specific chemical carcinogen-DNA interaction. Therefore, the authors sought to determine whether a relationship exists between PAH-DNA chemistry and chemically-induced genetic changes in end-stage liver tumors. Recently, they have demonstrated that a flounder c-Ki-{und ras} oncogene was present in NIH3T3 mouse fibroblasts transfected with DNA derived from a flounder liver tumor. Therefore, a more systematic study to detect oncogenes in affected flounder liver samples was undertaken. To characterize the oncogenes, DNA from the Nu-Nu tumors was isolated and analyzed by Southern blot hybridization of tumor DNA with a viral Ki-{und ras} DNA probe. This analysis revealed the presence of c-Ki-{und ras} oncogenes of fish origin in 6/7 (86%) of the Nu-Nu tumor DNAs analyzed. They hypothesize that common mechanisms of oncogene activation may be associated with tumor initiation. Using polymerase chain reaction (PCR) amplification and subsequent direct DNA sequence analysis of PCR-amplified DNA, they analyzed primary liver samples for the presence of DNA sequence changes in the first exon of the flounder c-Ki-{und ras} proto-oncogene. They have determined that a single G-C to T-A base transversion in the second position of the 12th codon (GLY to VAL) is present in a flounder sample which showed transforming activity in the Nu-Nu assay. This finding suggests that such a mutation may result in a transforming p21 protein in the flounder samples which were positive in the tumorgenicity assay.

• OSTI ID: 7164673
• Report Number(s): CONF-8909236--
• Conference Information: Journal Name: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA) Journal Volume: 28:2
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BODY
CARCINOGENESIS
CHEMICAL COMPOSITION
DIGESTIVE SYSTEM
DISEASES
DNA
DNA POLYMERASES
ENZYMES
FEDERAL REGION I
FISHES
GENES
GLANDS
HARBORS
HYBRIDIZATION
HYDROCARBONS
LIVER
MASSACHUSETTS
MUTATIONS
NEOPLASMS
NORTH AMERICA
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ONCOGENES
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHORUS-GROUP TRANSFERASES
POLYCYCLIC AROMATIC HYDROCARBONS
POLYMERASES
SENSITIVITY
TOXICITY
TRANSFERASES
USA
VERTEBRATES