Luminescence resonance energy transfer

Journal Article · Wed Jun 29 04:00:00 EDT 1994 · Journal of the American Chemical Society; (United States)

DOI:https://doi.org/10.1021/ja00092a088· OSTI ID:7164671

Selvin, P R; Rana, T M; Hearst, J E ^[1]

---

1. Univ. of California, Berkeley, CA (United States) Lawrence Berkeley Lab., CA (United States)

Fluorescence resonance energy transfer (FRET), in which a fluorescent donor molecule transfers energy via a nonradiative dipole-dipole interaction to an acceptor molecule (which is usually a fluorescent molecule), is a standard spectroscopic technique for measuring distances in the 10-70 Angstrom range. We have used a luminescent europium chelate as donor and an organic dye, CY-5, as acceptor. This luminescence resonance energy transfer (LRET) has several advantages over the more conventional FRET. The distance at which 50% of the energy is transferred (R[sub 0]) is large, 70 [angstrom]; the donor lifetime is single exponential and long (0.63 ms in H[sub 2]O; 2.5 ms in D[sub 2]O), making lifetime measurements facile and highly accurate; the orientation dependence (k[sup 2]) of energy transfer is minimized by the donor's multiple electronic transitions and long lifetime, limiting uncertainty in the measured distance due to orientation effects to [+-]12% in the worst case; the sensitized emission of the acceptor can be measured with little or no interfering background, yielding a >50-fold improvement in signal to background over standard donor-acceptor pairs and enabling distances several times R[sub 0] to be measured. 13 refs., 4 figs.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 7164671
• Journal Information: Journal of the American Chemical Society; (United States), Journal Name: Journal of the American Chemical Society; (United States) Vol. 116:13; ISSN JACSAT; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
400500* -- Photochemistry
BOSONS
COMPLEXES
DNA
DYES
ELEMENTARY PARTICLES
ENERGY TRANSFER
EUROPIUM COMPLEXES
FLUORESCENCE
HEAVY WATER
HYDROGEN COMPOUNDS
LUMINESCENCE
MASSLESS PARTICLES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOTONS
RARE EARTH COMPLEXES
SPECTROSCOPY
WATER