Effects of silicon deficiency on lipid composition and metabolism in the diatom Cyclotella cryptica

Journal Article · Thu Sep 01 04:00:00 EDT 1988 · Journal of Phycology; (USA)

DOI:https://doi.org/10.1111/j.1529-8817.1988.tb00189.x· OSTI ID:7164569

Roessler, P G ^[1]

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1. Solar Energy Research Institute, Golden, CO (USA)

The effects of silicon deficiency on the metabolism and composition of lipids in Cyclotella cryptica T13L Reimann, Lewin, and Guillard were examined Silicon-deficient cells had higher levels of neutral lipids (primarily triacylglycerols) and higher proportions of saturated and monounsaturated fatty acids than silicon-replete cells. After 4 h of silicon deficiency, the percentage of newly assimilated NaH{sup 14}CO{sub 3} partitioned into lipids increased from 27.6% to 54.1%, whereas the percentage partitioned into chrysolaminarin decreased from 21.6% to 54.1%, whereas the percentage partitioned into chrysolaminarin decreased from 21.6% to 10.6%. In addition, pulse-chase experiments with NaH{sup 14}CO{sub 3} indicated that the amount of {sup 14}C in the total cellular lipid fraction increased by 32% after 12 h of silicon deficiency despite the absence of additional photoassimilable {sup 14}C. Therefore, the accumulation of lipids in response to silicon deficiency appears to be due to two distinct processes: (1) an increase in the proportion of newly assimilated carbon partitioned into lipids, and (2) a slow conversion of previously assimilated carbon from non-lipid compounds into lipids.

• OSTI ID: 7164569
• Journal Information: Journal of Phycology; (USA), Journal Name: Journal of Phycology; (USA) Vol. 24:3; ISSN 0022-3646; ISSN JPYLA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
CHROMOPHYCOTA
DIATOMS
ELEMENTS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
METABOLISM
NUTRITIONAL DEFICIENCY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANKTON
PLANTS
SEMIMETALS
SILICON
TRACER TECHNIQUES