Structural and functional characterization of mouse U7 small nuclear RNA active in 3' processing of histone pre-mRNA

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.4.1518· OSTI ID:7164179

Soldati, D; Schumperli, D

Oligonucleotides derived from the spacer element of the histone RNA 3' processing signal were used to characterize mouse U7 small nuclear RNA (snRNA), i.e., the snRNA component active in 3' processing of histone pre-mRNA. Under RNase H conditions, such oligonucleotides inhibited the processing reaction, indicating the formation of a DNA-RNA hybrid with a functional ribonucleoprotein component. Moreover, these oligonucleotides hybridized to a single nuclear RNA species of approximately 65 nucleotides. The sequence of this RNA was determined by primer extension experiments and was found to bear several structural similarities with sea urchin U7 snRNA. The comparison of mouse and sea urchin U7 snRNA structure yields some further insight into the mechanism of histone RNA 3' processing.

• Research Organization: Institut fur Molekularbiologie II der Universitat Zurich, Honggerberg, CH-8093 Zurich (CH)
• OSTI ID: 7164179
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:4; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
DATA
DNA
EXPERIMENTAL DATA
HISTONES
HYBRIDIZATION
INFORMATION
MAMMALS
MESSENGER-RNA
MICE
NUCLEIC ACIDS
NUCLEOPROTEINS
NUMERICAL DATA
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PROTEINS
RNA
RNA PROCESSING
RODENTS
STRUCTURE-ACTIVITY RELATIONSHIPS
VERTEBRATES