Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumontae

Patent · Tue Oct 02 00:00:00 EDT 1990

OSTI ID:7162961

Lacks, S A

Chromosomal DNA cassettes containing genes encoding either the DpnI or DpnII restriction endonucleases from Streptococcus pneumoniae are cloned into a streptococcal vector, pLS101. Large amounts of the restriction enzymes are produced by cells containing the multicopy plasmids, pLS202 and pLS207, and their derivatives pLS201, pLS211, pLS217, pLS251 and pLS252. 9 figs.

• DOE Contract Number: AC02-76CH00016
• Assignee: Associated Universities, Inc., Washington, DC (United States)
• Patent Number(s): US 4960707; A
• Application Number: PPN: US 7-085944
• OSTI ID: 7162961
• Resource Relation: Patent File Date: 17 Aug 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
STREPTOCOCCUS
GENETIC ENGINEERING
DNA-CLONING
ENDONUCLEASES
RECOMBINANT DNA
BACTERIA
BIOTECHNOLOGY
CLONING
DNA
DNA HYBRIDIZATION
DNA-ASE
ENZYMES
ESTERASES
HYBRIDIZATION
HYDROLASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROTEINS
550200* - Biochemistry