Biochemical and mutagenic characterization of plant-activated aromatic amines

Journal Article · Sun Aug 01 04:00:00 EDT 1993 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1002/etc.5620120805· OSTI ID:7161983

Plewa, M J; Gichner, T; Xin, H; Seo, K Y; Smith, S R; Wagner, E D ^[1]

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1. Univ. of Illinois, Urbana, IL (United States)

Plant activation is the process by which promutagenic agents are activated into mutagens by plant systems. With the widespread use of agricultural chemicals on crop plants and with the global exposure of plants to pollutants, the possibility exists that plant-activated agents may be introduced into the human food chain. The plant cell/microbe coincubation assay uses cultured plant cell suspensions as the activating system and bacteria or yeast cells as the genetic indicator organism. After a treatment time, the microbes are plated on selective medium. In this way the activation system and the genetic system can be independently studied. In addition, the viability of the plant cells and the microbial cells can be independently determined so that the toxicity of a test agent can be evaluated. Using cytochrome P450 monooxygenase and peroxidase inhibitors, the authors are studying the biochemical mechanisms of plant activation of environmental contaminants, especially aromatic amines. They propose a working model for the activation of aromatic amines by cultured tobacco cells into stable frameshift mutagens.

• OSTI ID: 7161983
• Journal Information: Environmental Toxicology and Chemistry; (United States), Journal Name: Environmental Toxicology and Chemistry; (United States) Vol. 12:8; ISSN 0730-7268; ISSN ETOCDK
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
BIOCHEMICAL REACTION KINETICS
CONTAMINATION
FOOD CHAINS
KINETICS
METABOLISM
MUTAGENESIS
ORGANIC COMPOUNDS
REACTION KINETICS