Characterization of the cDNA and genomic sequence of a G protein [gamma] subunit ([gamma][sub 5])
- Pennsylvania State Univ., University Park, PA (United States)
A cDNA from human placenta and liver tissues that contained both sequence for the lysosomal glycosidase di-N-acetylchitobiase and sequence homologous to the subunit of GTP-binding proteins was previously isolated. Here, we have shown that the [gamma]-subunit-homologous portion of this unusual cDNA is derived from a member of the [gamma]-subunit multigene family. The partial human [gamma]-subunit sequence was used to isolate the corresponding full-length cDNA clones from bovine and rat livers. The two cDNAs encode identical 68-amino-acid proteins (7.3 kDa) homologous to previously cloned G protein [gamma] subunits. The bovine gene sequence encoding this new [gamma]-subunit isoform ([gamma][sub 5]) was determined and found to have an intron-exon structure consistent with the original human chitobiase-[gamma][sub 5]-subunit hybrid mRNA being a product of alternative splicing. Genomic cloning also resulted in the isolation of a human [gamma][sub 5] pseudogene. 25 refs., 7 figs.
- OSTI ID:
- 7160604
- Journal Information:
- Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 12:4; ISSN 0270-7306; ISSN MCEBD4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Isolation and characterization of human cDNA clones encoding the. alpha. and the. alpha. prime subunits of casein kinase II
Distinct forms of the. beta. subunit of GTP-binding regulatory proteins identified by molecular cloning