Sequence of the human iduronate 2-sulfatase (IDS) gene

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1406· OSTI ID:7160489

Wilson, P J; Meaney, C A; Hopwood, J J; Morris, C P ^[1]

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1. Adelaide Children's Hospital, North Adelaide (Australia)

Deficiency of the lysosomal enzyme iduronate-2-sulfatase (IDS; EC 3.1.6.13) results in the storage of the glycosaminoglycans heparan sulfate and dermatan sulfate, which leads to the lysosomal storage disorder mucopolysaccharidosis type II. Three overlapping genomic clones derived from an X-chromosome-specific library containing the entire IDS gene were isolated and the sequences of the intron boundaries and the 5[prime] promoter region were determined. The IDS gene is split into nine exons spanning approximately 24 kb. The potential promoter for IDS lacks a TATA box but contains GC box consensus sequences, consistent with its role as a housekeeping gene. A polypyrimidine-like repeat is found in intron 1. 9 refs., 1 fig., 1 tab.

• OSTI ID: 7160489
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:3; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINES
CARBOHYDRATES
CHROMOSOMES
DISEASES
DNA SEQUENCING
ENZYMES
GENES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
METABOLIC DISEASES
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
X CHROMOSOME