Isolation of the cDNA and chromosomal localization of the gene (TAX1) encoding the human axonal glycoprotein TAG-1

Journal Article · Wed Dec 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80357-X· OSTI ID:7160241

Tsiotra, P C; Karagogeos, D; Theodorakis, K; Michaelidis, T M; Papamatheakis, J ^[1]; Modi, W S ^[2]; Furley, A J; Jessell, T M ^[3]

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1. Univ. of Crete (Greece)
2. Frederick Cancer Research and Development Center, MD (United States)
3. Columbia Univ., New York, NY (United States)

The transient axonal glycoprotein (TAG-1) is a cell adhesion molecule that promotes neurite outgrowth and belongs to the immunoglobulin superfamily. The authors have isolated cDNAs encoding TAX1, the human homologue of TAG-1. Human TAX1 shows a high degree of homology to rat TAX1 and less to its chick counterpart, axonin-1, with 91 and 75% identity at the amino acid level, respectively. The numbers of immunoglobulin (IgC2) domains and fibronectin repeats present in TAG-1 are conserved among the three species. The highest degree of conservation occurs in the second IgC2 domain (98% with the rat and 82% with the chick). The human homologue also contains a putative N-terminal signal sequence and a C-terminal hydrophobic sequence, suggestive of linkage to the cell membrane via phosphatidylinositol. In addition, the two mammalian TAG-1 proteins share the RDG tripeptide, a motif known to mediate recognition of fibronection by integrins. In situ hybridization to human metaphase chromosomes maps the TAX1 gene encoding human TAG-1 to a single location on chromosome 1q32. 35 refs., 3 figs.

• OSTI ID: 7160241
• Journal Information: Genomics; (United States), Vol. 18:3; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ADHESION
GENES
DNA SEQUENCING
HUMAN CHROMOSOME 1
GENETIC MAPPING
MEMBRANE PROTEINS
NERVE CELLS
GROWTH
DNA-CLONING
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics