Frequent NF2 gene transcript mutations in sporadic meningiomas and vestibular schwannomas

Journal Article · Wed Jun 01 04:00:00 EDT 1994 · American Journal of Human Genetics; (United States)

OSTI ID:7160082

Deprez, R H.L.; Groen, N A; Zwarthoff, E C; Hagemeijer, A; Van Drunen, E; Bootsma, D; Koper, J W; Avezaat, C J.J. ^[1]; Bianchi, A B; Seizinger, B R ^[2]

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1. Erasmus Univ., Rotterdam (Netherlands)
2. Bristol Myers-Squibb Pharmaceutical Research Institute, Princeton, NY (United States)

The gene for the hereditary disorder neurofibromatosis type 2 (NF2), which predisposes for benign CNS tumors such as vestibular schwannomas and meningiomas, has been assigned to chromosome 22 and recently has been isolated. Mutations in the NF2 gene were found in both sporadic meningiomas and vestibular schwannomas. However, so far only 6 of the 16 exons of the gene have been analyzed. In order to extend the analysis of an involvement of the NF2 gene in the sporadic counterparts of these NF2-related tumors, the authors have used reverse transcriptase-PCR amplification followed by SSCP and DNA sequence analysis to screen for mutations in the coding region of the NF2 gene. Analysis of the NF2 gene transcript in 53 unrelated patients with meningiomas and vestibular schwannomas revealed mutations in 32% of the sporadic meningiomas (n = 44), in 50% of the sporadic vestibular schwannomas (n = 4), in 100% of the tumors found in NF2 patients (n = 2), and in one of three tumors from multiple-meningioma patients. Of the 18 tumors in which a mutation in the NF2 gene transcript was observed and the copy number of chromosome 22 could be established, 14 also showed loss of (parts of) chromosome 22. This suggests that in sporadic meningiomas and NF2-associated tumors the NF2 gene functions as a recessive tumor-suppressor gene. The mutations detected resulted mostly in frameshifts, predicting truncations starting within the N-terminal half of the putative protein. 23 refs., 2 figs. 3 tabs.

• OSTI ID: 7160082
• Journal Information: American Journal of Human Genetics; (United States), Journal Name: American Journal of Human Genetics; (United States) Vol. 54:6; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CENTRAL NERVOUS SYSTEM
DETECTION
DISEASES
DNA SEQUENCING
GENE AMPLIFICATION
GENE MUTATIONS
MUTATIONS
NEOPLASMS
NERVOUS SYSTEM
POLYMERASE CHAIN REACTION
STRUCTURAL CHEMICAL ANALYSIS