Sequence and genetic organization of a Zymomonas mobilis gene cluster that encodes several enzymes of glucose metabolism

Journal Article · Sat Dec 01 04:00:00 EST 1990 · Journal of Bacteriology; (United States)

OSTI ID:7160054

Barnell, W O; Yi, Kyung Cheol; Conway, T ^[1]

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1. Univ. of Nebraska, Lincoln (United States)

The Zymomonas mobilis genes that encode glucose-6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), and glucokinase (glk) were cloned independently by genetic complementation of specific defects in Escherichia coli metabolism. The identify of these cloned genes was confirmed by various biochemical means. Nucleotide sequence analysis established that these three genes are clustered on the genome and revealed an additional open reading frame in this region that has significant amino acid identity to the E.coli xylose-proton symporter and the human glucose transporter. On the basis of this evidence and structural analysis of the deduced primary amino acid sequence, this gene is believed to encode the Z. mobilis glucose-facilitated diffusion protein, glf. The four genes in the 6-kb cluster are organized in the order glf, zwf, edd, glk. The glf and zwf genes are separated by 146 bp. The zwf and edd genes overlap by 8 bp, and their expression may be translationally coupled. The edd and glk genes are separated by 203 bp. The glk gene is followed by tandem transcriptional terminators. The four genes appear to be organized in an operon. Such an arrangement of the genes that govern glucose uptake and the first three steps of the Entner-Doudoroff glycolytic pathway provides the organism with a mechanism for carefully regulating the levels of the enzymes that control carbon flux into the pathway.

• DOE Contract Number: FG02-90ER20002
• OSTI ID: 7160054
• Journal Information: Journal of Bacteriology; (United States), Journal Name: Journal of Bacteriology; (United States) Vol. 172:12; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINO ACID SEQUENCE
BACTERIA
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CLONING
ENZYMES
GENE OPERONS
GENES
GLUCOSE
HEXOSES
MEMBRANES
METABOLISM
MICROORGANISMS
MOLECULAR STRUCTURE
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
SACCHARIDES
TRANSFERASES
ZYMOMONAS MOBILIS