Specific and quantitative assessment of naphthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium
- Univ. of Tennessee, Knoxville (United States)
A bioassay was developed and standardized for the rapid, specific, and quantitative assessment of naphthalene and salicylate bioavailability by use of bioluminescence monitoring of catabolic gene expression. The bioluminescent reporter strain Pseudomonas fluorescens HK44, which carries a transcriptional nahG-luxCDABE fusion for naphthalene and salicylate catabolism, was used. The physiological state of the reporter cultures as well as the intrinsic regulatory properties of the naphthalene degradation operon must be taken into account to obtain a high specificity at low target substrate concentrations. Experiments have shown that the use of exponentially growing reporter cultures has advantages over the use of carbon-starved, resting cultures. In aqueous solutions for both substrates, naphthalene and salicylate, linear relationships between initial substrate concentration and bioluminescence response were found over concentration ranges of 1 to 2 orders of magnitude. Naphthalene could be detected at a concentration of 45 ppb. Studies conducted under defined conditions with extracts and slurries of experimentally contaminated sterile soils and identical uncontaminated soil controls demonstrated that this method can be used for specific and quantitative estimations of target pollutant presence and bioavailability in soil extracts and for specific and qualitative estimations of napthalene in soil slurries.
- DOE Contract Number:
- FG05-91ER61193
- OSTI ID:
- 7159746
- Journal Information:
- Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:6; ISSN AEMIDF; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Rapid, sensitive bioluminescent reporter technology for napthalene exposure and biodegradation
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIOASSAY
BIODEGRADATION
BIOLOGICAL AVAILABILITY
BIOLUMINESCENCE
CARBOXYLIC ACIDS
CATABOLISM
CELL CULTURES
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
GROWTH
HYDROCARBONS
HYDROXY ACIDS
LUMINESCENCE
METABOLISM
MICROORGANISMS
NAPHTHALENE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PSEUDOMONAS
SALICYLIC ACID
SOILS