Integrin (. alpha. sub 6 /. beta. sub 4 ) expression in human lung cancer as monitored by specific monoclonal antibodies
- Univ. di Chieti (Italy)
- Regina Elena Cancer Inst., Rome (Italy)
- Oak Ridge National Lab., TN (United States)
In this study, the expression of the {alpha}{sub 6}/{beta}{sub 4} integrin complex was analyzed in human lung carcinomas both in vitro and in vivo, using two monoclonal antibodies which recognize the integrin subunits {alpha}{sub 6} (Mab 135-13C) and {beta}{sub 4} (Mab 439-9B). Immunoprecipitation patterns obtained from established human lung carcinoma cell lines demonstrated that the {alpha}{sub 4} and the {beta}{sub 4} subunits were differentially expressed in carcinomas of different types. The {alpha}{sub 6} subunit was expressed in all the cell lines tested. The {beta}{sub 4} subunit was expressed in non-small cell cancer lines but was not detectable in the small cell cancer line tested. Using a quantitative two-site assay, the authors measured the concentration of the {alpha}{sub 6}/{beta}{sub 4} integrin in matched biopsies from primary lung tumors and from normal lung. These studies confirmed that the complex was differentially expressed in non-small versus small cell lung cancers and that it was also detectable in lysates from normal lung at low levels. The highest levels of {alpha}{sub 6}/{beta}{sub 4} were found in moderately differentiated squamous cell carcinomas. By immunohistochemistry, the {beta}{sub 4} subunit was detectable in all the squamous cell carcinomas and adenocarcinomas tested but not in 10 small cell cancers. The patterns of immunoreactivity were consistent with the expected distribution of membrane glycoproteins and, in some squamous cell carcinomas, were suggestive of the localization displayed by molecules involved in carcinoma-stroma interaction. Immunohistochemical staining indicating that {beta}{sub 4} was also expressed in specific types of nonrespiratory pulmonary epithelial cells.
- OSTI ID:
- 7159743
- Journal Information:
- Cancer Research; (United States), Vol. 50; ISSN 0008-5472
- Country of Publication:
- United States
- Language:
- English
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