Ethanol reassimilation and ethanol tolerance in Pitchia stipitis CBS 6054 as studied by [sup 13]C nuclear magnetic resonance spectroscopy
Journal Article
·
· Applied and Environmental Microbiology; (United States)
OSTI ID:7159709
- Univ. of Lund (Sweden)
- Univ. of Odense (Denmark)
Ethanol reassimilation in Pichia stipitis CBS 6054 was studied by using continuous cultures, and the oxidation of [1-[sup 13]C] ethanol was monitored by in vivo and in vitro [sup 13]C nuclear magnetic resonance spectroscopy. Acetate was formed when ethanol was reassimilated. The ATP/ADP ratio and the carbon dioxide production decreased, whereas the malate dehydrogenase activity increased, in ethanol-reassimilating cells. The results are discussed in terms of the low ethanol tolerance in P. stipitis compared with that in Saccharomyces cerevisiae.
- OSTI ID:
- 7159709
- Journal Information:
- Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:8; ISSN AEMIDF; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
ALCOHOLS
ATP
CARBON 13
CARBON ISOTOPES
COMPARATIVE EVALUATIONS
CONTINUOUS CULTURE
ENZYME ACTIVITY
ENZYMES
ETHANOL
EUMYCOTA
EVALUATION
EVEN-ODD NUCLEI
FUNGI
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
ISOTOPES
LIGHT NUCLEI
METABOLISM
MICROORGANISMS
NMR SPECTRA
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
PROTEINS
SPECTRA
STABLE ISOTOPES
TOLERANCE
TOXICITY
YEASTS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
ALCOHOLS
ATP
CARBON 13
CARBON ISOTOPES
COMPARATIVE EVALUATIONS
CONTINUOUS CULTURE
ENZYME ACTIVITY
ENZYMES
ETHANOL
EUMYCOTA
EVALUATION
EVEN-ODD NUCLEI
FUNGI
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
ISOTOPES
LIGHT NUCLEI
METABOLISM
MICROORGANISMS
NMR SPECTRA
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
PROTEINS
SPECTRA
STABLE ISOTOPES
TOLERANCE
TOXICITY
YEASTS