Infusion and sampling site effects on two-pool model estimates of leucine metabolism

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Am. J. Physiol.; (United States)

OSTI ID:7154386

Helland, S J; Grisdale-Helland, B; Nissen, S

To assess the effect of site of isotope infusion on estimates of leucine metabolism infusions of alpha-(4,5-3H)ketoisocaproate (KIC) and (U-/sup 14/C)leucine were made into the left or right ventricles of sheep and pigs. Blood was sampled from the opposite ventricle. In both species, left ventricular infusions resulted in significantly lower specific radioactivities (SA) of (/sup 14/C)leucine and (/sup 3/H)KIC. (/sup 14/C)KIC SA was found to be insensitive to infusion and sampling sites. (/sup 14/C)KIC was in addition found to be equal to the SA of (/sup 14/C)leucine only during the left heart infusions. Therefore, (/sup 14/C)KIC SA was used as the only estimate for (/sup 14/C)SA in the equations for the two-pool model. This model eliminated the influence of site of infusion and blood sampling on the estimates for leucine entry and reduced the impact on the estimates for proteolysis and oxidation. This two-pool model could not compensate for the underestimation of transamination reactions occurring during the traditional venous isotope infusion and arterial blood sampling.

• Research Organization: Iowa State Univ., Ames (USA)
• OSTI ID: 7154386
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 254; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DOMESTIC ANIMALS
HEART
ISOTOPE APPLICATIONS
KETO ACIDS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
MATERIALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RUMINANTS
SHEEP
SWINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES