Activation of bone marrow phagocytes following benzene treatment of mice
- Rutgers Univ., Piscataway, NJ (USA)
Techniques in flow cytometry/cell sorting were used to characterize the effects of benzene and its metabolites on subpopulations of bone marrow cells. Treatment of male Balb/c mice with benzene or a combination of its metabolites, hydroquinone and phenol, resulted in a 30 to 40% decrease in bone marrow cellularity. Flow cytometric analysis revealed two subpopulations of bone marrow cells that could be distinguished by their size and density or granularity. The larger, more dense subpopulation was found to consist predominantly of macrophages and granulocytes as determined by monoclonal antibody binding and by cell sorting. Benzene treatment had no selective cytotoxic effects on subpopulations of bone marrow cells. To determine if benzene treatment activated bone marrow phagocytes, the authors quantified production of hydrogen peroxide by these cells using the fluorescent indicator dye, 2{prime},7{prime}-dichlorofluorescin diacetate. They found that macrophages and granulocytes from bone marrow of treated mice produced 50% more hydrogen peroxide in response to the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate than did cells from control animals. It is hypothesized that phagocyte activation and production of cytotoxic reactive oxygen intermediates may contribute to hematotoxicity induced by benzene.
- OSTI ID:
- 7150329
- Journal Information:
- Environmental Health Perspectives; (USA), Journal Name: Environmental Health Perspectives; (USA) Vol. 82; ISSN 0091-6765; ISSN EVHPA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
AROMATICS
BENZENE
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BONE MARROW CELLS
CARCINOGENS
CELL FLOW SYSTEMS
CHEMICAL ACTIVATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
ESTERS
HYDROCARBONS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROXY COMPOUNDS
MAMMALS
METABOLITES
MICE
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PHAGOCYTOSIS
PHENOL
PHENOLS
PHORBOL ESTERS
QUINONES
RODENTS
SOMATIC CELLS
VERTEBRATES