Method of synthesizing double-stranded DNA molecules

Patent · Wed Nov 08 04:00:00 EST 1989

OSTI ID:7147415

Jones, D

This invention relates to a method in which the polymerase chain reaction (PCR) is used to generate products that when combined, denatured, and reannealed, form double-stranded DNA with discrete, cohesive, single-stranded ends. Such cohesive ends can be utilized in the formation of precise, directional DNA joints without dependence on enzyme-restriction sites. Furthermore, the placement of cohesive single strands on both ends of a DNA molecule permits a DNA strand or strands to be annealed to form circular DNA. These recombinant circles of DNA can be directly transfected into appropriate host cells. The method of generating recombinant circles upon reannealing PCR generated products simplifies DNA mutagenesis, recombination, and cloning.

• Research Organization: National Insts. of Health, Bethesda, MD (USA)
• Assignee: National Institutes of Health, Bethesda, MD
• Patent Number(s): A US 7-432993
• OSTI ID: 7147415
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CELL CONSTITUENTS
DNA
DNA POLYMERASES
ENZYMES
KINETICS
MOLECULES
MUTATIONS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLASMIDS
POLYMERASES
REACTION KINETICS
SYNTHESIS
TRANSFERASES