Studies on DNA replication in vitro by DNA polymerase-. alpha. /primase complex from embryonic chicken brain
Thesis/Dissertation
·
OSTI ID:7147073
DNA polymerase - {alpha} activity, characterized by its sensitivity to N-ethylmaleimide, high sedimentation coefficient, and acidic isoelectric point was found to decline with increasing embryonic age. Primase activity, the enzyme responsible for the initiation of DNA synthesis, was found to co-sediment with DNA polymerase - {alpha} activity on a continuous glycerol velocity gradient. Of all the single-stranded DNA templates tested, primase activity was found to be maximally active with poly dC. In the presence of ({alpha}-{sup 32}P) GTP, primase activity was found to catalyze the formation of an alkali-labile oligoriboguanylate, 15-20 nucleotides long. The chain length of the products were not altered by the presence or absence of dGTP. Primase activity was not inhibited by a high concentration of {alpha}- amanitin. Consistent with the finding of a decline of the activity level of DNA polymerase - {alpha}, the level of DNA polymerase - {alpha} antigen was also found to decrease with embryonic age, as evidenced by ELISA with a non-neutralizing monoclonal antibody, SJK 237-71.
- Research Organization:
- Notre Dame Univ., IN (USA)
- OSTI ID:
- 7147073
- Country of Publication:
- United States
- Language:
- English
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Fri May 01 00:00:00 EDT 1987
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·
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
BIRDS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHICKENS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DNA POLYMERASES
DNA REPLICATION
EMBRYOS
ENZYME ACTIVITY
ENZYME IMMUNOASSAY
ENZYMES
FOWL
IMIDES
IMMUNOASSAY
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
RNA POLYMERASES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
BIRDS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHICKENS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DNA POLYMERASES
DNA REPLICATION
EMBRYOS
ENZYME ACTIVITY
ENZYME IMMUNOASSAY
ENZYMES
FOWL
IMIDES
IMMUNOASSAY
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
RNA POLYMERASES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES